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Benchling Inc crispr guide rna design tool
Crispr Guide Rna Design Tool, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crispr+design+tool/crispr+design+tool/pmc13155868-62-34-40
Average 86 stars, based on 1 article reviews
crispr guide rna design tool - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Cloning:

Article Title: Saturation editing of RNU4-2 reveals distinct dominant and recessive disorders.
Article Snippet: .. Single guide RNA design and cloning The gRNA used for SGE was designed using Benchling’s CRISPR design tool to search the RNU4-2 locus, including upstream and downstream regions of low sequence homology to RNU4-1 and pseudogenes, identifying a candidate with high on-target and low off-target scores. ..

CRISPR:

Article Title: Saturation editing of RNU4-2 reveals distinct dominant and recessive disorders.
Article Snippet: .. Single guide RNA design and cloning The gRNA used for SGE was designed using Benchling’s CRISPR design tool to search the RNU4-2 locus, including upstream and downstream regions of low sequence homology to RNU4-1 and pseudogenes, identifying a candidate with high on-target and low off-target scores. ..

Article Title: Phorbol Myristate Acetate Inhibits Senecavirus A Replication by Activating IKBKE–Mediated IFN Pathway and NF‐κB Signal
Article Snippet: .. Three sgRNA sequences were designed using an online CRISPR design tool (Benchling, https://www.benchling.com/crispr ). ..

Article Title: PD‐L1‐Binding Antigen Presenters: Redirecting Vaccine‐Induced Antibodies for Cancer Immunotherapy
Article Snippet: .. The sgRNA sequences targeting mouse (sgRNA: GTATGGCAG‐CAACGTCACGA) and human PD‐L1 (sgRNA: ACCGTTCAGCAAATGCCAGT)were designed using the online CRISPR design tool (Benchling) and transfected into 4T1 cells and MDA‐MB‐231 cells using Lipofectamine 3000 (Invitrogen) separately. ..

Article Title: A repressive regulatory cascade shapes temporal patterning of activity-regulated gene expression in a defined sensory neuron type
Article Snippet: .. crRNAs, tracrRNAs and Cas9 protein were obtained from Integrated DNA Technologies (IDT). crRNAs were designed using Benchling’s built in CRISPR design tool. ..

Article Title: PD-L1-Binding Antigen Presenters: Redirecting Vaccine-Induced Antibodies for Cancer Immunotherapy.
Article Snippet: .. The sgRNA sequences targeting mouse (sgRNA: GTATGGCAG-CAACGTCACGA) and human PD-L1 (sgRNA: ACCGTTCAGCAAATGCCAGT)were designed using the online CRISPR design tool (Benchling) and transfected into 4T1 cells and MDA-MB-231 cells using Lipofectamine 3000 (Invitrogen) separately. ..

Article Title: The lysosomal LAMTOR-Rag complex functions as a checkpoint for antiviral interferon production
Article Snippet: CRISPR-Cas9-mediated knockout cell line generation was performed using pLentiCRISPRv2-GFP-puro, a construct modified from pLentiCRISPRv2-puro (Addgene #52961). .. Small guide RNAs (sgRNAs) were selected using the CRISPR design tool of Benchling or CHOPCHOP v3. ..

Article Title: Pmel17 Deficiency Affects Melanogenesis and Promotes Tumor Vascularization
Article Snippet: .. The single guide RNA (sgRNA) was designed using Benchling’s CRISPR design tool ( https://www.benchling.com ) accessed on 23 November 2020. .. The designed sequences (5′-CACCGCACTCAGCACAAGCACGGGA-3′ and 5′-AAACTCCCGTGCTTGTGCTGAGTGC-3′) were phosphorylated, annealed, and cloned into the BbsI-digested pSpCas9(BB)-2A-Puro (PX459) V2.0 plasmid (Addgene #62988) [ ].

Sequencing:

Article Title: Saturation editing of RNU4-2 reveals distinct dominant and recessive disorders.
Article Snippet: .. Single guide RNA design and cloning The gRNA used for SGE was designed using Benchling’s CRISPR design tool to search the RNU4-2 locus, including upstream and downstream regions of low sequence homology to RNU4-1 and pseudogenes, identifying a candidate with high on-target and low off-target scores. ..

Transfection:

Article Title: PD‐L1‐Binding Antigen Presenters: Redirecting Vaccine‐Induced Antibodies for Cancer Immunotherapy
Article Snippet: .. The sgRNA sequences targeting mouse (sgRNA: GTATGGCAG‐CAACGTCACGA) and human PD‐L1 (sgRNA: ACCGTTCAGCAAATGCCAGT)were designed using the online CRISPR design tool (Benchling) and transfected into 4T1 cells and MDA‐MB‐231 cells using Lipofectamine 3000 (Invitrogen) separately. ..

Article Title: PD-L1-Binding Antigen Presenters: Redirecting Vaccine-Induced Antibodies for Cancer Immunotherapy.
Article Snippet: .. The sgRNA sequences targeting mouse (sgRNA: GTATGGCAG-CAACGTCACGA) and human PD-L1 (sgRNA: ACCGTTCAGCAAATGCCAGT)were designed using the online CRISPR design tool (Benchling) and transfected into 4T1 cells and MDA-MB-231 cells using Lipofectamine 3000 (Invitrogen) separately. ..

Multiple Displacement Amplification:

Article Title: PD‐L1‐Binding Antigen Presenters: Redirecting Vaccine‐Induced Antibodies for Cancer Immunotherapy
Article Snippet: .. The sgRNA sequences targeting mouse (sgRNA: GTATGGCAG‐CAACGTCACGA) and human PD‐L1 (sgRNA: ACCGTTCAGCAAATGCCAGT)were designed using the online CRISPR design tool (Benchling) and transfected into 4T1 cells and MDA‐MB‐231 cells using Lipofectamine 3000 (Invitrogen) separately. ..

Article Title: PD-L1-Binding Antigen Presenters: Redirecting Vaccine-Induced Antibodies for Cancer Immunotherapy.
Article Snippet: .. The sgRNA sequences targeting mouse (sgRNA: GTATGGCAG-CAACGTCACGA) and human PD-L1 (sgRNA: ACCGTTCAGCAAATGCCAGT)were designed using the online CRISPR design tool (Benchling) and transfected into 4T1 cells and MDA-MB-231 cells using Lipofectamine 3000 (Invitrogen) separately. ..

other:

Article Title: Pmel17 Deficiency Affects Melanogenesis and Promotes Tumor Vascularization.
Article Snippet: The B16F10 murine melanoma cell line was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).



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